产品名称：Stemgent/Human Recombinant BMP-4/03-0007/10 μg

产品编号：03-0007

市场价：¥0.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>细胞因子>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：待定

品牌： Stemgent

公司：Stemgent, Inc

公司分类：

商品介绍

ProductOverview

BoneMorphogeneticProtein4(BMP-4)isapolypeptidebelongingtotheTGF-βproteinsuper-family.BMP-4isinvolvedinboneandcartilagedevelopment;morespecifically,intoothandlimbdevelopmentfracturerepair¹.Inhumanembryonicdevelopment,BMP-4isacriticalsignalingmoleculerequiredfortheearlydifferentiationoftheembryoandestablishmentofadorsal-ventralaxis^2,3.BMP-4playsanimportantroleindifferentiationofoverlyingectodermaltissue.InhibitionoftheBMP-4signalcausestheectodermtodifferentiateintotheneuralplate.Inculturedstemcells,BMP-4playsadistinctroleinmouseandhumanembryonicstem(ES)cells.BMP-4supportsLIFasapositivefactorformouseEScellself-renewal⁴.Incontrast,BMP-4inducesextra-embryonictrophoblastdifferentiationinhumanEScells⁵. StemfactorBMP-4isarecombinantproteinexpressedandpurifiedfromhuman293cellsasaglycosylatedhomodimerwithamolecularmassof34kDa.

ProductSpecifications

Size

10µg

Source

StemfactorBMP-4wasexpressedinandpurifiedfromhuman293cells.

Formulation

Lyophilizedfromsterile-filtered50mMNaOAc,pH4.5and1MNaCl.

AminoAcidSequence

SPKHHSQRARKKNKNCRRHSLYVDFSDVGWNDWIVAPPGYQAFYCHGDCPFPLADHLNSTNHAIVQTLVNSVNSSIPKACCVPTELSAISMLYLDEYDKVVLKNYQEMVVEGCGCR

UniprotAccessionNumber

P12644,residues293-408

Purity

Greaterthan95%bySDS-PAGEanalysis.

EndotoxinLevel

Lessthan1.0EU/μgofBMP-4asdeterminedbytheLALmethod.

BIOLOGicActivity

TheED₅₀islessthan30ng/mlasdeterminedbyitsABIlitytoinducealkalinephosphataseproductionbymousechondrogenicATDC-5cells.

Sterility

TestedtobenegativeforMycoplasmasp.byPCRandmicrobialcontaminationbya sterilitytest.

StorageandStability

StemfactorBMP-4isshippedatroomtemperature.LyophilizedBMP-4isstableforupto6monthsfromdateofreceiptwhenstoredat-20°Cto-80°C.ReconstitutedBMP-4,atconcentrationsgreaterthanorequalto0.1mg/ml,isstableforupto3monthswhenstoredat-20°Candupto6monthswhenstoredat-80°C.

Reconstitution

CentrifugebrieflyandthenreconstituteBMP-4in4mMHCltoyieldastocksolutionofnolessthan0.1mg/ml.Avoidfreeze-thawcyclesasitcanresultinlossofactivity.

SpecificationSheets

03-0007SpecificationsSheet

SafetyDataSheets

03-0007SafetyDataSheet

References

Miljkovic,N.D.,Cooper,G.M.,andMarra,K.G.(2008)Chondrogenesis,bonemorphogeneticprotein-4andmesenchymalstemcells.OsteoarthritisCartilage16:1121-1130.
Chen,D.,Zhao,M.,andMundy,G.R.(2004)Bonemorphogeneticproteins.GrowthFactors22:233-241.
Sadlon,T.J.,Lewis,I.D.,andD’Andrea,R.J.(2004)BMP4:itsroleindevelopmentofthehematopoieticsystemandpotentialasahematopoieticgrowthfactor.StemCells22:457-474.
Ying,Q.L.,Nichols,J.,Chambers,I.,andSmith,A.(2003)BMPinductionofIdproteinssuppressesdifferentiationandsustainsembryonicstemcellself-renewalincollaborationwithSTAT3.Cell115:281-292.
Xu,R.H.,Chen,X.,Li,D.S.,Li,R.,Addicks,G.C.,Glennon,C.,Zwaka,T.P.,andThompson,J.A.(2002)BMP4initiateshumanembryonicstemcelldifferentiationtotrophoblasts.NatBiotechnol.20:1261-1264.

AdditionalPublications

SaxenaS;RonnRE;GuibentifC;MoraghebaR;WoodsN-B."CyclicAMPsignalingthroughEpacaxismodulateshumanhemogenicendotheliumandenhanceshematopoeiticcellgeneration."StemCellRepdoi:10.1016/j.stemcr.2016.03.006(2016)
QianX."Definingthemechanismbywhichsyntheticpolymersurfacessupporthumanpluripotentstemcellself-renewal."PhDThesis,UniversityofMichigan(2015)
ZhuS;WangH;DengS."Reprogrammingfibroblaststowardcardiomyocytes,neuralstemcellsandhepatocytesbycellactivationandsignaling-directedlineageconversion."NatureProtocols10,959-973(2015)
Villa-DiazLG;KimJK;LahannJ;KresbachPH."Derivationandlong-termcultureoftransfene-freehumaninducedpluripotentstemcellsonsyntheticsubstates."StemCellsTransMed3:1410(2014)
ChiangP-M;WongPC."Differentiationofanembryonicstemcelltohemogenicendotheliumbydefinedfactors:Essentialroleofbonemorphogeneticprotein4."Development138:2833-2843(2011)
Wang,B.,Miyagoe-Suzuki,Y.,Yada,E.,Ito,N.,Nishiyama,T.,Nakamura,M.,Ono,Y.,Motohashi,N.,Segawa,M.,Masuda,S.,Takeda,S.ReprogrammingEfficiencyandQualityofInducedPluripotentStemCells(iPSCs)GeneratedfromMuscle-derivedFibroblastsofMDXMiceatDifferentAges.PLOSCurrentsMuscularDystrophy.2011Oct27.Edition1.doi:10.1371/currents.RRN1274.

品牌介绍

Stemgent mRNA体细胞重编程系统

产品特点

1.快速 Fast

Stemgent® MicroRNA-Enhanced mRNA Reprogramming System只需2周即可将靶细胞重编程为iPS细胞，与仙台病毒（Sendai Virus）、非整合型DNA载体（Episomal DNA Vectors）等传统iPS重编程方法相比，缩短实验周期超过50%以上（Figure. 1）。

Figure 1. Stemgent® mRNA重编程系统、仙台病毒依赖的重编程系统以及游离型DNA载体依赖的重编程系统重编程时程比较。

简便 Easy

与病毒、DNA载体依赖的iPS重编程手段不同，使用Stemgent® MicroRNA-Enhanced mRNA Reprogramming System对靶细胞进行重编程，生成的iPS细胞无需进行复杂耗时的下游筛选程序，只需简简单单6个步骤，即可轻松构建iPS细胞系。

	Description	Day
Step 1	Material Preparation	Prior to starting
Step 2	Plate Cells	0
Step 3	Transfect Cells	1 to 12
Step 4	Identify Cells	13-14
Step 5	Pick and Passage iPS Cell Colonies	15-16
Step 6	Maintain iPS Cell Cultures	16+

注：因所用细胞类型及实验条件不同，上述时间轴可能略有不同，仅供参考。

Figure 2. Stemgent® MicroRNA-Enhanced mRNA Reprogramming System重编程过程时间表、显微镜下观察到的重编程过程中靶细胞形态变化。第12天，形成的iPS克隆表达多能型Marker TRA-1-81。

高效 Efficient

病毒、DNA载体等依赖的iPS重编程技术，其重编程效率介于0.00001%到1%之间不等，而Stemgent® MicroRNA-Enhanced mRNA Reprogramming System的重编程效率远远大于1%，极大地提高了iPS克隆的产出率。

表1. 不同类型iPS重编程手段效率比较

Reprogramming Method	Efficiency	Integrating	Screening
RNA	>1%	No	No
仙台病毒（Sendai virus）	0.01-1%	No	Yes
DNA载体（Episomal/Minicircle）	0.00%	Possible	Yes
慢病毒（Lentivirus）	0.001-0.01%	Yes	Yes
腺病毒（Adenovirus）	0.0001-0.001%	Possible	Yes
Protein	0.00%	No	No

安全 Safe

某些iPS研究领域，例如iPS临床研究、治疗应用等，对iPS细胞的安全性提出了极高的要求。使用Stemgent® MicroRNA-Enhanced mRNA Reprogramming System重编程靶细胞，无需担心构建的iPS细胞系可能含有病毒载体残留，亦无需担心无关的基因整合进宿主基因组，是目前适用于临床研究及治疗的最理想选择。

而以仙台病毒（Sendai Virus）为载体的iPS重编程手段，其病毒基因组虽然不会整合进宿主染色体，仙台病毒本身也不具备复制能力，但是为了构建真正意义上的virus-free的iPS细胞系，通常需要经过一个10-20代、耗时费力的筛选过程，以确保最终得到的iPS细胞系无病毒载体残留。以慢病毒（Lentivirus）体的iPS重编程手段，病毒基因组能够永久整合进宿主基因组，会给后续的iPS细胞分析带来干扰。